development of a new immunochromatographic assay using gold nanoparticles for screening of iga deficiency.
نویسندگان
چکیده
a new competitive immunochromatography (icg) strip test based on polyclonal antibody (pab) conjugated with gold nanoparticles (nps) was developed and its applications for primary screening of immunoglobulin (ig) a in serum were evaluated. nanocolloidal gold as the detection reagent, with an average particle diameter of 20 nm, was synthesized and labelled pab. the antibody-nanocolloidal gold probe was applied on the conjugate pad, and human iga was immobilized on a nitrocellulose membrane as the capture reagent to prepare the icg strip test. it took only 10 minutes to accomplish a semi-quantitative detection of serum iga in this assay. in the optimized investigational conditions, the icg strip test could distinguish human serum iga in the range from 1 to 270 ng/ml with a detection limit of 5 ng/ml. the reliability of testing procedures was examined by performing the icg strip test with 11 serum samples and comparing the results with those obtained via enzyme-linked immunosorbent assay (elisa). the icg strip was sufficiently sensitive and accurate for a rapid screening of iga in human serum.
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عنوان ژورنال:
iranian journal of allergy, asthma and immunologyجلد ۱۴، شماره ۱، صفحات ۱۰۵-۱۱۲
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